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A human milk factor susceptible to cathepsin D inhibitors enhances human immunodeficiency virus type 1 infectivity and allows virus entry into a mammary epithelial cell line.

Authors
  • El Messaoudi, K
  • Thiry, L F
  • Liesnard, C
  • Van Tieghem, N
  • Bollen, A
  • Moguilevsky, N
Type
Published Article
Journal
Journal of virology
Publication Date
Jan 01, 2000
Volume
74
Issue
2
Pages
1004–1007
Identifiers
PMID: 10623764
Source
Medline
License
Unknown

Abstract

Human immunodeficiency virus type 1 (HIV-1) growth in lymphocyte cultures was increased when the virus inoculum was incubated in breast milk. The enhancing effect of milk was abolished by anti-cathepsin D antibody or by pepstatin A, a cathepsin D inhibitor. The cathepsin D-producing CD4-negative MCF7 mammary cells supported the growth of some HIV-1 isolates. An MCF7 line chronically producing HIV-1 IIIb was obtained. Cathepsin D may induce conformational modification of viral gp120, allowing direct interaction with a coreceptor. We demonstrated the presence of CXCR4 mRNA in MCF7 cells.

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