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Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells.

Authors
  • R G Goodwin
  • S Lupton
  • A Schmierer
  • K J Hjerrild
  • R Jerzy
  • W Clevenger
  • S Gillis
  • D Cosman
  • A E Namen
Publication Date
Jan 01, 1989
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kDa for the mature protein. Recombinant human interleukin 7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells. Analysis of RNA by blot hybridization demonstrated the presence of two size classes of interleukin 7 mRNA in human splenic and thymic tissue.

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