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Human Chorionic Gonadotropin Enhances Trophoblast–Epithelial Interaction in an In Vitro Model of Human Implantation

Authors
  • Racicot, Karen E.1
  • Wünsche, Vera1
  • Auerbach, Ben1
  • Aldo, Paulomi1
  • Silasi, Michelle1
  • Mor, Gil1, 2
  • 1 Yale University School of Medicine, 333 Cedar St LSOG 305A, New Haven, CT, 06520, USA , New Haven (United States)
  • 2 Yale University School of Medicine, New Haven, CT, USA , New Haven (United States)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Oct 01, 2014
Volume
21
Issue
10
Pages
1274–1280
Identifiers
DOI: 10.1177/1933719114522553
Source
Springer Nature
Keywords
License
Yellow

Abstract

Embryo implantation, which is an absolute requirement for reproduction, starts with blastocyst apposition to the uterine endometrium, followed by attachment to the endometrial surface epithelium. Recent clinical studies reported an increase in implantation and pregnancy rates among women receiving intrauterine human chorionic gonadotropin (hCG) prior to embryo transfer suggesting that, at least in some cases, female infertility is a result of inadequate secretion of hCG. In this study, we characterized the effect of hCG on trophoblast–epithelial interaction by further developing our recently described in vitro model of implantation. Here, we confirmed hCG increased attachment of trophoblast to epithelial cells, using a single-cell trophoblast–epithelial coculture system in addition to a blastocyst-like spheroid–epithelial coculture system. Furthermore, we discovered that the source and concentration was pivotal; the first preparation of hCG affected 2 molecules related to implantation, MUC16 and osteopontin, while the second preparation required additional cytokines to mimic the effects. Using this system, we can develop a comprehensive knowledge of the cellular and gene targets of hCG and other factors involved in embryo apposition and implantation and potentially increase the number of therapeutic targets for subfertile patients.

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