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Human blood IgM "memory" B cells are circulating splenic marginal zone B cells harboring a prediversified immunoglobulin repertoire.

Authors
  • Weller, Sandra
  • Braun, Moritz C
  • Tan, Bruce K
  • Rosenwald, Andreas
  • Cordier, Corinne
  • Conley, Mary Ellen
  • Plebani, Alessandro
  • Kumararatne, Dinakhanta S
  • Bonnet, Damien
  • Tournilhac, Olivier
  • Tchernia, Gil
  • Steiniger, Birte
  • Staudt, Louis M
  • Casanova, Jean-Laurent
  • Reynaud, Claude-Agnès
  • Weill, Jean-Claude
Type
Published Article
Journal
Blood
Publisher
American Society of Hematology
Publication Date
Dec 01, 2004
Volume
104
Issue
12
Pages
3647–3654
Identifiers
PMID: 15191950
Source
Medline
License
Unknown

Abstract

The human peripheral B-cell compartment displays a large population of immunoglobulin M-positive, immunoglobulin D-positive CD27(+) (IgM(+)IgD(+)CD27(+)) "memory" B cells carrying a mutated immunoglobulin receptor. By means of phenotypic analysis, complementarity-determining region 3 (CDR3) spectratyping during a T-independent response, and gene-expression profiling of the different blood and splenic B-cell subsets, we show here that blood IgM(+)IgD(+)CD27(+) cells correspond to circulating splenic marginal zone B cells. Furthermore, analysis of this peripheral subset in healthy children younger than 2 years shows that these B cells develop and mutate their immunoglobulin receptor during ontogeny, prior to their differentiation into T-independent antigen-responsive cells. It is therefore proposed that these IgM(+)IgD(+)CD27(+) B cells provide the splenic marginal zone with a diversified and protective preimmune repertoire in charge of the responses against encapsulated bacteria.

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