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Human 6-phosphofructo-1-kinase gene has an additional intron upstream of start codon.

Authors
  • Valdez, B C
  • Chen, Z
  • Sosa, M G
  • Younathan, E S
  • Chang, S H
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
Mar 15, 1989
Volume
76
Issue
1
Pages
167–169
Identifiers
PMID: 2526044
Source
Medline
License
Unknown

Abstract

A 6-phosphofructo-1-kinase-coding gene (pfk) has been purified from a human genomic library cloned in the lambda EMBL4 phage vector. This clone contains the nontranslated 5' flanking region of the human muscle pfk gene. Comparison of the nucleotide sequence determined by us with that of the human muscle pfk cDNA [Nakajima et al., FEBS Lett. 223 (1987) 113-116] indicates the presence of an additional intron extending from nucleotide (nt) -97 to -9 upstream of the ATG start codon. Furthermore, the human muscle pfk gene is more AT-rich than the rabbit gene. The available sequence of the two cDNAs shows 256 nt differences. Surprisingly, 71% of these sites are A's and T's in the human cDNA and C's and G's in the rabbit gene.

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