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HSF1-Dependent Autophagy Activation Contributes to the Survival of Melanocytes Under Oxidative Stress in Vitiligo.

Authors
  • Cui, Tingting1
  • Wang, Yinghan1
  • Song, Pu1
  • Yi, Xiuli1
  • Chen, Jiaxi1
  • Yang, Yuqi1
  • Wang, Huina1
  • Kang, Pan1
  • Guo, Sen1
  • Liu, Ling1
  • Li, Kai1
  • Jian, Zhe1
  • Li, Shuli1
  • Li, Chunying2
  • 1 Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. , (China)
  • 2 Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Journal of Investigative Dermatology
Publisher
Elsevier
Publication Date
Jun 01, 2022
Volume
142
Issue
6
Identifiers
DOI: 10.1016/j.jid.2021.11.007
PMID: 34780715
Source
Medline
Language
English
License
Unknown

Abstract

Autophagy plays a protective role in oxidative stress‒induced melanocyte death. Dysregulated autophagy increases the sensitivity of melanocytes in response to oxidative damage and promotes melanocyte degeneration in vitiligo. However, the molecular mechanism underlying this process is not fully understood. In this study, using RNA-sequencing technology, we compared the transcriptome change between normal and vitiligo melanocytes with or without treatment of oxidative stress. We found that ATG5 and ATG12, the critical components for autophagosome formation, were significantly reduced in vitiligo melanocytes under oxidative stress. Mechanistically, HSF1 is the prime transcription factor for both ATG5 and ATG12, accounting for the reduced level of ATG5 and ATG12 in vitiligo melanocytes. Deficiency of HSF1 led to accumulation of intracellular ROS, imbalance of mitochondrion membrane potential, and apoptosis in melanocytes exposure to oxidative stress. Furthermore, overexpression of HSF1 could ameliorate oxidative stress‒induced melanocytes death through the activation of autophagy by upregulating ATG5 and ATG12. These findings suggested that targeting HSF1-ATG5/12 axis could prevent oxidative stress‒induced melanocyte death and may be used as a therapeutic strategy for vitiligo treatment. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

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