Affordable Access

deepdyve-link
Publisher Website

Hsa_circ_0000515 is a novel circular RNA implicated in the development of breast cancer through its regulation of the microRNA-296-5p/CXCL10 axis.

Authors
  • Cai, Fenglin1
  • Fu, Wenjie2
  • Tang, Lei1
  • Tang, Jinhai3
  • Sun, Jinming4
  • Fu, Guangshun4
  • Ye, Gang4
  • 1 Department of General Surgery, Clinical Medical College of Yangzhou University & Northern Jiangsu People's Hospital, Yangzhou, China. , (China)
  • 2 Department of General Surgery, Jiangsu Provincial Corps Hospital of Chinese People's Armed Police Forces, Yangzhou, China. , (China)
  • 3 Department of General Surgery, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China. , (China)
  • 4 Department of General Surgery, Jiangdu People's Hospital of Yangzhou, Yangzhou, China. , (China)
Type
Published Article
Journal
FEBS Journal
Publisher
Wiley (Blackwell Publishing)
Publication Date
Feb 01, 2021
Volume
288
Issue
3
Pages
861–883
Identifiers
DOI: 10.1111/febs.15373
PMID: 32446265
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Cancer metastasis is a major cause of death among women afflicted with breast cancer (BC) and understanding the molecular processes involved is a major focus in BC research. Circular RNAs (circRNAs) have emerged as genomic regulatory molecules in carcinogenesis and metastasis; however, their role in BC is unclear. We characterized a novel circRNA, hsa_circ_0000515, in context of BC. We collected 340 cancerous tissues surgically resected from BC patients and found hsa_circ_0000515 was upregulated in BC tissues and associated with poor prognosis of BC. Silencing of hsa_circ_0000515 impaired cell cycle progression, cell proliferation, and invasion, attenuated inflammatory response, and reduced the proangiogenetic potential of BC cells. RNA pull-down and dual-luciferase reporter gene assays showed that hsa_circ_0000515 binds miR-296-5p, preventing it from repressing CXCL10 expression. We also observed that miR-296-5p inhibition or CXCL10 overexpression promoted cell cycle progression, restored proliferative, invasive and proangiogenetic abilities, and increased inflammatory response in MCF-7 cells in the absence of hsa_circ_0000515. In vivo analyses showed that partial loss of hsa_circ_0000515 reduced the tumor growth of MCF-7 cells in nude mice. The key findings from this study revealed that targeting hsa_circ_0000515 might be an effective strategy to combat BC. © 2020 Federation of European Biochemical Societies.

Report this publication

Statistics

Seen <100 times