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An HPLC-PAD method to analyse flavonoid glycosides and styrylpyrones from Cryptocarya species (Lauraceae).

Authors
  • Nehme, Cláudia J
  • Bastos, Wagner L
  • de Araújo, Adelson J
  • Cavalheiro, Alberto J
Type
Published Article
Journal
Phytochemical analysis : PCA
Publication Date
Jan 01, 2005
Volume
16
Issue
2
Pages
93–97
Identifiers
PMID: 15881116
Source
Medline
License
Unknown

Abstract

An HPLC-PAD method has been developed in order to evaluate simultaneously the main secondary metabolites, flavonoid glycosides and styrylpyrones, of leaves of Cryptocarya moschata. The sample preparation, consisting of extraction, liquid-liquid extraction and centrifugation, requires minimum sample manipulation but produces high yields with reproducibility, selectivity and simplicity. HPLC on a C18 column presents each class of metabolites grouped and with good resolution of the main compounds. The experimental conditions can be used to study inter- and intra-specific variability of secondary metabolites in Cryptocarya spp.

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