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HPLC-DAD-MS identification of bioactive secondary metabolites from Ferula communis roots.

Authors
  • Arnoldi, Lolita
  • Ballero, Mauro
  • Fuzzati, Nicola
  • Maxia, Andrea
  • Mercalli, Enrico
  • Pagni, Luca
Type
Published Article
Journal
Fitoterapia
Publisher
Elsevier
Publication Date
Jun 01, 2004
Volume
75
Issue
3-4
Pages
342–354
Identifiers
PMID: 15158993
Source
Medline
License
Unknown

Abstract

A simple HPLC method was developed to distinguish between 'poisonous' and 'non-poisonous' chemotypes of Ferula communis. The method was performed on a C8 reverse phase analytical column using a binary eluent (aqueous TFA 0.01%-TFA 0.01% in acetonitrile) under gradient condition. The two chemotypes showed different fingerprints. The identification of five coumarins and eleven daucane derivatives by HPLC-diode array detection (HPLC-DAD) and HPLC-MS is described. A coumarin, not yet described, was detected.

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