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How Cells Tiptoe on Adhesive Surfaces before Sticking

Authors
  • Pierres, Anne1, 1, 1, 1
  • Benoliel, Anne-Marie1, 1, 1, 1
  • Touchard, Dominique1, 1, 1, 1
  • Bongrand, Pierre1, 1, 1, 1
  • 1 7, 13288 Marseille cedex 09, France
Type
Published Article
Journal
Biophysical Journal
Publisher
Elsevier
Publication Date
Jan 30, 2008
Volume
94
Issue
10
Pages
4114–4122
Identifiers
DOI: 10.1529/biophysj.107.125278
PMID: 18234815
PMCID: PMC2367202
Source
PubMed Central
License
Unknown

Abstract

Cell membranes are studded with protrusions that were thoroughly analyzed with electron microscopy. However, the nanometer-scale three-dimensional motions generated by cell membranes to fit the topography of foreign surfaces and initiate adhesion remain poorly understood. Here, we describe the dynamics of surface deformations displayed by monocytic cells bumping against fibronectin-coated surfaces. We observed membrane undulations with typically 5 nm amplitude and 5–10 s lifetime. Cell membranes behaved as independent units of micrometer size. Cells detected the presence of foreign surfaces at 50 nm separation, resulting in time-dependent amplification of membrane undulations. Molecular contact then ensued with apparent cell-membrane separation of 30–40 nm, and this distance steadily decreased during the following tens of seconds. Contact maturation was associated with in-plane egress of bulky molecules and robust membrane fluctuations. Thus, membrane undulations may be the major determinant of cell sensitivity to substrate topography, outcome of interaction, and initial kinetics of contact extension.

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