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Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568

Authors
  • Gajdos, Tamás1
  • Hopp, Béla1
  • Erdélyi, Miklós1
  • 1 University of Szeged,
Type
Published Article
Journal
Journal of Fluorescence
Publisher
Springer-Verlag
Publication Date
Feb 29, 2020
Volume
30
Issue
3
Pages
437–443
Identifiers
DOI: 10.1007/s10895-020-02496-0
PMID: 32112289
PMCID: PMC7250809
Source
PubMed Central
Keywords
License
Unknown

Abstract

Hot-band absorption and anti-Stokes emission properties of an organic fluorescent dye, Alexa Fluor 568, were characterized and compared with those of Rhodamine 101. The comparison of the properties (e.g., quantum efficiency, spectral distribution, thermal properties, and fluorescence lifetime) between the two dyes confirms that both dyes undergo the same process when excited in the red spectral region. Possible undesirable crosstalk effects and applications in dSTORM microscopy were demonstrated and discussed.

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