In Tunisia, we observed that rotavirus P-3 and P in young children with gastroenteritis associate with the secretor histo-blood-group phenotype. Inversely, the emerging P-4, representing 10% of cases, was exclusively found among nonsecretor patients. Unlike VP8* from P-3 and P strains, the P-4 VP8* protein attached to glycans from saliva samples regardless of the donors Secretor status. Interestingly, a high frequency of FUT2 enzyme deficiency (nonsecretor phenotype) was observed in the population. This may allow co-circulation of P-3 and P-4 strains in secretor and nonsecretor children, respectively.