Affordable Access

Host RNA polymerase requirements for transcription of the human hepatitis delta virus genome.

Authors
  • Moraleda, G
  • Taylor, J
Type
Published Article
Journal
Journal of virology
Publication Date
Nov 01, 2001
Volume
75
Issue
21
Pages
10161–10169
Identifiers
PMID: 11581384
Source
Medline
License
Unknown

Abstract

Replication of the genome of hepatitis delta virus (HDV) requires RNA-directed RNA synthesis using a host polymerase(s). This manuscript reviews the relevant published evidence. It also provides two new studies, both of which made use of transiently transfected Huh7 cells undergoing HDV RNA-directed RNA synthesis. For the first study, RNA transcription inhibitors were added to the transfected cells for periods of 1 to 2 days, after which assays of the effects on the accumulation of processed unit-length genomic HDV RNA were performed. For the second study, nuclei were isolated at 6 days after transfection, and then in vitro runoff transcription was used to assay the effects of RNA transcription inhibitors. Overall, the data support the interpretation that HDV transcription does not require host polymerase I or III (pol I or III) but at least primarily involves an enzyme resembling pol II.

Report this publication

Statistics

Seen <100 times