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The horizontal gene transfer of Agrobacterium T-DNAs into the series Batatas (Genus Ipomoea) genome is not confined to hexaploid sweetpotato

Authors
  • Quispe-Huamanquispe, Dora G.1, 2
  • Gheysen, Godelieve1
  • Yang, Jun3
  • Jarret, Robert4
  • Rossel, Genoveva2
  • Kreuze, Jan F.2
  • 1 Ghent University, Department of Biotechnology, Ghent, 9000, Belgium , Ghent (Belgium)
  • 2 International Potato Center (CIP), Lima, Peru , Lima (Peru)
  • 3 Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai, China , Shanghai (China)
  • 4 USDA-ARS/PGRU, Griffin, GA, 30223, USA , Griffin (United States)
Type
Published Article
Journal
Scientific Reports
Publisher
Springer Nature
Publication Date
Aug 29, 2019
Volume
9
Issue
1
Identifiers
DOI: 10.1038/s41598-019-48691-3
Source
Springer Nature
License
Green

Abstract

The discovery of the insertion of IbT-DNA1 and IbT-DNA2 into the cultivated (hexaploid) sweetpotato [Ipomoea batatas (L.) Lam.] genome constitutes a clear example of an ancient event of Horizontal Gene Transfer (HGT). However, it remains unknown whether the acquisition of both IbT-DNAs by the cultivated sweetpotato occurred before or after its speciation. Therefore, this study aims to evaluate the presence of IbT-DNAs in the genomes of sweetpotato’s wild relatives belonging to the taxonomic group series Batatas. Both IbT-DNA1 and IbT-DNA2 were found in tetraploid I. batatas (L.) Lam. and had highly similar sequences and at the same locus to those found in the cultivated sweetpotato. Moreover, IbT-DNA1 was also found in I. cordatotriloba and I. tenuissima while IbT-DNA2 was detected in I. trifida. This demonstrates that genome integrated IbT-DNAs are not restricted to the cultivated sweetpotato but are also present in tetraploid I. batatas and other related species.

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