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A homology model of restriction endonuclease SfiI in complex with DNA.

Authors
  • Chmiel, Agnieszka A
  • Bujnicki, Janusz M
  • Skowronek, Krzysztof J
Type
Published Article
Journal
BMC Structural Biology
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jan 01, 2005
Volume
5
Pages
2–2
Identifiers
PMID: 15667656
Source
Medline
License
Unknown

Abstract

The bioinformatics analysis suggest that SfiI, a Type IIF enzyme, is more closely related to BglI, an "orthodox" Type IIP restriction enzyme, than to any other REase, including other Type IIF REases with known structures, such as NgoMIV. NgoMIV and BglI belong to two different, very remotely related branches of the PD-DxK superfamily: the alpha-class (EcoRI-like), and the beta-class (EcoRV-like), respectively. Thus, our analysis provides evidence that the ability to tetramerize and cut the two DNA sequences in a concerted manner was developed independently at least two times in the evolution of the PD-DxK superfamily of REases. The model of SfiI will also serve as a convenient platform for further experimental analyses.

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