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Histone deacetylase inhibitors and cell proliferation in pea root meristems.

Authors
  • Murphy, J P
  • McAleer, J P
  • Uglialoro, A
  • Papile, J
  • Weniger, J
  • Bethelmie, F
  • Tramontano, W A
Type
Published Article
Journal
Phytochemistry
Publisher
Elsevier
Publication Date
Sep 01, 2000
Volume
55
Issue
1
Pages
11–18
Identifiers
PMID: 11021638
Source
Medline
License
Unknown

Abstract

The histone deacetylase (HDA) inhibitors, trichostatin A (TSA) and HC toxin halt mitosis in cultured root meristems of Pisnum sativum, while the anti-protozoan HDA inhibitor apicidin is ineffective. Two-dimensional PAGE of proteins from root meristems exposed to TSA and HC toxin did not show significant differences compared to controls, although a previously tested HDA inhibitor, butyrate, exhibited dramatic variations in its protein profile. Northern analysis of butyrate- and TSA-treated root meristems indicated that non-proliferating cells are expressing significant amounts of transcripts of the known cell proliferation associated genes: histone H2A, MAP kinase, cycA2:1 and cdc2. Western analysis reveals the presence of hyperacetylated nuclear proteins in HDA-inhibitor treated cells. These results suggest that the HDA inhibitors, butyrate and TSA, halt mitosis without down-regulating genes that typically have low or nonexistent expression levels in non-dividing cells.

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