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Histomorphometry of the ligaments using a generic-purpose image processing software, a new strategy for semi-automatized measurements.

Authors
  • Ballesteros, Rafael
  • Bonsfills, Nuria
  • Chacón, Marta
  • García-Lázaro, Javier
  • Gómez-Barrena, Enrique
Type
Published Article
Journal
Journal of Digital Imaging
Publisher
Springer-Verlag
Publication Date
Aug 01, 2012
Volume
25
Issue
4
Pages
527–536
Identifiers
DOI: 10.1007/s10278-011-9440-8
PMID: 22143412
Source
Medline
License
Unknown

Abstract

Gold chloride technique can be combined with Adobe Photoshop® software to yield a quantitative assessment of the different areas in heterogeneous structures as are ligament. A semi-automatized method based on the sum of two- and three-dimensional morphological criteria upon colorimetric criteria allows the identification and measurement of the area occupied by a structure of interest. It also allows the quantification of color intensity to differentiate structures with similar staining avidity, like vessels and nerves. This computer-assisted, semiquantitative procedure for computerized morphometry is relatively simple to perform. The accuracy, efficiency, and reproducibility of this method based on a commercially available imaging program were considered adequate when tested on the anterior cruciate ligament of the cat. Image normalization by trained observers using a commercially available software package designed for photography, applied to a sample randomly chosen, has provided the means of making reproducible measurements of heterogeneous structures.

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