Affordable Access

Access to the full text

Hippocampal plasticity involves extensive gene induction and multiple cellular mechanisms

Authors
  • Hevroni, Dana1
  • Rattner, Amir1
  • Bundman, Marsha1
  • Lederfein, Doron1
  • Gabarah, Awni1
  • Mangelus, Miriam1
  • Silverman, Michael A.1
  • Kedar, Hilla1
  • Naor, Cathy1
  • Kornuc, Masayo2
  • Hanoch, Tamar3
  • Seger, Rony3
  • Theill, Lars E.2
  • Nedivi, Elly1
  • Richter-Levin, Gal4
  • Citri, Yoav1
  • 1 The Weizmann Institute of Science, Department of Hormone Research, Rehovot, 76100, Israel , Rehovot
  • 2 Amgen Center, Molecular Biology Department, Thousand Oaks, CA, 91320-1789 , Thousand Oaks
  • 3 The Weizmann Institute of Science, Membrane Research and Biophysics, Rehovot, 76100, Israel , Rehovot
  • 4 University of Haifa, Department of Psychology, Haifa, 31905, Israel , Haifa
Type
Published Article
Journal
Journal of Molecular Neuroscience
Publisher
Springer-Verlag
Publication Date
Apr 01, 1998
Volume
10
Issue
2
Pages
75–98
Identifiers
DOI: 10.1007/BF02737120
Source
Springer Nature
Keywords
License
Yellow

Abstract

Long-term plasticity of the central nervous system (CNS) involves induction of a set of genes whose identity is incompletely characterized. To identify candidate plasticity-related genes (CPGs), we conducted an exhaustive screen for genes that undergo induction or downregulation in the hippocampus dentate gyrus (DG) following animal treatment with the potent glutamate analog, kainate. The screen yielded 362 upregulated CPGs and 41 downregulated transcripts (dCPGs). Of these, 66 CPGs and 5 dCPGs are known genes that encode for a variety of signal transduction proteins, transcription factors, and structural proteins. Seven novel CPGs predict the following putative functions:cpg2—a dystrophin-like cytoskeletal protein;cpg4—a heat-shock protein:cpg16—a protein kinase;cpg20—a transcription factor;cpg21—a dual-specificity MAP-kinase phosphatase; andcpg30 andcpg38—two new seven-transmembrane domain receptors. Experiments performed in vitro and with cultured hippocampal cells confirmed the ability of thecpg-21 product to inactivate the MAP-kinase. To test relevance to neural plasticity, 66 CPGs were tested for induction by stimuli producing long-term potentiation (LTP). Approximately one-fourth of the genes examined were upregulated by LTP. These results indicate that an extensive genetic response is induced in mammalian brain after glutamate receptor activation, and imply that a significant proportion of this activity is coinduced by LTP. Based on the identified CPGs, it is conceivable that multiple cellular mechanisms underlie long-term plasticity of the nervous system.

Report this publication

Statistics

Seen <100 times