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Hin recombinase bound to DNA: the origin of specificity in major and minor groove interactions.

Authors
  • Feng, J A
  • Johnson, R C
  • Dickerson, R E
Type
Published Article
Journal
Science (New York, N.Y.)
Publication Date
Jan 21, 1994
Volume
263
Issue
5145
Pages
348–355
Identifiers
PMID: 8278807
Source
Medline
License
Unknown

Abstract

The structure of the 52-amino acid DNA-binding domain of the prokaryotic Hin recombinase, complexed with a DNA recombination half-site, has been solved by x-ray crystallography at 2.3 angstrom resolution. The Hin domain consists of a three-alpha-helix bundle, with the carboxyl-terminal helix inserted into the major groove of DNA, and two flanking extended polypeptide chains that contact bases in the minor groove. The overall structure displays features resembling both a prototypical bacterial helix-turn-helix and the eukaryotic homeodomain, and in many respects is an intermediate between these two DNA-binding motifs. In addition, a new structural motif is seen: the six-amino acid carboxyl-terminal peptide of the Hin domain runs along the minor groove at the edge of the recombination site, with the peptide backbone facing the floor of the groove and side chains extending away toward the exterior. The x-ray structure provides an almost complete explanation for DNA mutant binding studies in the Hin system and for DNA specificity observed in the Hin-related family of DNA invertases.

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