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High-throughput peptide derivatization toward supramolecular diversification in microtiter plates

  • Lin, Y
  • Penna, M
  • Spicer, CD
  • Higgins, SG
  • Gelmi, A
  • Kim, N
  • Wang, S-T
  • Wojciechowski, JP
  • Pashuck, ET
  • Yarovsky, I
  • Stevens, MM
Publication Date
Feb 05, 2021
UPCommons. Portal del coneixement obert de la UPC
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The evolution of life on earth eventually leads to the emergence of species with increased complexity and diversity. Similarly, evolutionary chemical space exploration in the laboratory is a key step to pursue the structural and functional diversity of supramolecular systems. Here, we present a powerful tool that enables rapid peptide diversification and employ it to expand the chemical space for supramolecular functions. Central to this strategy is the exploitation of palladium-catalyzed Suzuki-Miyaura cross-coupling reactions to direct combinatorial synthesis of peptide arrays in microtiter plates under an open atmosphere. Taking advantage of this in situ library design, our results unambiguously deliver a fertile platform for creating a set of intriguing peptide functions including green fluorescent protein-like peptide emitters with chemically encoded emission colors, hierarchical self-assembly into nano-objects, and macroscopic hydrogels. This work also offers opportunities for quickly surveying the diversified peptide arrays and thereby identifying the structural factors that modulate peptide properties.

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