A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses
- Authors
- Type
- Published Article
- Journal
- Cell Reports
- Publisher
- Elsevier
- Publication Date
- Jul 28, 2020
- Volume
- 32
- Issue
- 4
- Identifiers
- DOI: 10.1016/j.celrep.2020.107968
- PMID: 32726631
- PMCID: PMC7408500
- Source
- PubMed Central
- Keywords
- License
- Unknown
Abstract
Holderith et al. present a sensitive, quantitative immunolocalization method that allows the visualization of dozens of synaptic proteins in epoxy-resin-embedded ultrathin sections with a resolution of <40 nm. Their method is ideal for molecular characterization of individual synapses following in vitro paired recordings, two-photon [Ca2+] or glutamate-sensor (iGluSnFR) imaging.
