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A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses

Authors
  • Holderith, Noemi1
  • Heredi, Judit1
  • Kis, Viktor1
  • Nusser, Zoltan1
  • 1 Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Budapest 1083, Hungary
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Jul 28, 2020
Volume
32
Issue
4
Identifiers
DOI: 10.1016/j.celrep.2020.107968
PMID: 32726631
PMCID: PMC7408500
Source
PubMed Central
Keywords
License
Unknown

Abstract

Holderith et al. present a sensitive, quantitative immunolocalization method that allows the visualization of dozens of synaptic proteins in epoxy-resin-embedded ultrathin sections with a resolution of <40 nm. Their method is ideal for molecular characterization of individual synapses following in vitro paired recordings, two-photon [Ca2+] or glutamate-sensor (iGluSnFR) imaging.

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