Affordable Access

deepdyve-link
Publisher Website

A highly sensitive signal-amplified gold nanoparticle-based electrochemical immunosensor for dibutyl phthalate detection.

Authors
  • Liang, Ya-Ru1
  • Zhang, Zong-Mian2
  • Liu, Zhen-Jiang1
  • Wang, Kun3
  • Wu, Xiang-Yang1
  • Zeng, Kun1
  • Meng, Hui1
  • Zhang, Zhen4
  • 1 School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. , (China)
  • 2 School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: [email protected] , (China)
  • 3 The School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. , (China)
  • 4 School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Biosensors & bioelectronics
Publication Date
May 15, 2017
Volume
91
Pages
199–202
Identifiers
DOI: 10.1016/j.bios.2016.12.007
PMID: 28011415
Source
Medline
Keywords
License
Unknown

Abstract

A simple and signal-amplified, label-free electrochemical impedimetric immunosensor for dibutyl phthalate (DBP), a type of phthalate ester, was developed using gold nanoparticles (AuNPs) induced signal amplification. The approach was based on an indirectly competitive binding system of coating antigen that coated on antigen/chitosan/[email protected]/GCE (modified electrodes), target (DBP) and anti-DBP antibody. Signal amplification was performed via the enlargement of AuNPs through NADH-promoted catalytic precipitation, which caused a marked increase in the electron-transfer resistance and electrostatic repulsion after an AuNP-labeled second antibody conjugated to anti-DBP. Under the optimized conditions, this method displayed: (i) low detection limits (7ng/mL, 10 times lower than the traditional ELISA method using the same antibody); (ii) satisfactory accuracy (recoveries, 86.0-120.4%) and agreement with corresponding ELISA method; (iii) high tolerance to some environmental interferents; and (iv) low cost and low sample consumption (6µL). Our results demonstrate the great potential and high efficiency of this immunosensor in practical applications for the cost-effective and sensitive monitoring of DBP in aquatic environments.

Report this publication

Statistics

Seen <100 times