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Highly sensitive quantitative analysis of nicotianamine using LC/ESI-TOF-MS with an internal standard.

Authors
  • Wada, Yasuaki
  • Yamaguchi, Isomaro
  • Takahashi, Michiko
  • Nakanishi, Hiromi
  • Mori, Satoshi
  • Nishizawa, Naoko K
Type
Published Article
Journal
Bioscience, biotechnology, and biochemistry
Publication Date
Feb 01, 2007
Volume
71
Issue
2
Pages
435–441
Identifiers
PMID: 17284859
Source
Medline
License
Unknown

Abstract

A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of N(epsilon)-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA.

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