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Higher nisin yield is reached with glutathione and pyruvate compared with heme in Lactococcus lactis N8.

Authors
  • Girgin Ersoy, Zeynep1
  • Kayıhan, Ceyhun2
  • Tunca, Sedef3
  • 1 Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University, Gebze, Kocaeli, Turkey. , (Turkey)
  • 2 Department of Molecular Biology and Genetics, Faculty of Science and Letters, Başkent University, Etimesgut, Ankara, Turkey. , (Turkey)
  • 3 Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University, Gebze, Kocaeli, Turkey. [email protected] , (Turkey)
Type
Published Article
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Publication Date
Sep 01, 2020
Volume
51
Issue
3
Pages
1247–1257
Identifiers
DOI: 10.1007/s42770-019-00216-w
PMID: 31898248
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

There are different studies that aim to enhance the production of nisin by Lactococcus lactis since its chemical synthesis is not possible. In this study, glutathione (GSH) and pyruvate, which are known to reduce the oxidative stress of cells, have been shown to trigger the production of nisin at both transcriptional and translational levels in L. lactis cells grown under aerobic condition. Presence of GSH and pyruvate caused more nisin yield than the heme-supplemented medium. Moreover, the expression of genes that encode stress-related enzymes were apparently upregulated in the presence of GSH and pyruvate. It can be concluded that GSH and pyruvate contribute to the defense system of L. lactis cells and so that higher biomass was obtained which in turn enhance nisin production. Antioxidant effect of GSH and pyruvate was known; however, their stimulating effect on nisin production was shown for the first time in this study.

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