High throughput approaches to study RNA-protein interactions in vitro.
Center for RNA Science and Therapeutics, School of Medicine, Case Western Reserve University, Cleveland, OH, United States.
Center for RNA Science and Therapeutics, School of Medicine, Case Western Reserve University, Cleveland, OH, United States. Electronic address: [email protected]
- Published Article
- Publication Date
Jun 01, 2020
To understand the regulation of gene expression it is critical to determine how proteins interact with and discriminate between different RNAs. In this review, we discuss experimental techniques that utilize high throughput approaches to characterize the interactions of proteins with large numbers of RNAs in vitro. We describe the underlying principles for the main methods, briefly discuss their scope and limitations, and outline how insight from the techniques contributes to our understanding of specificity for RNA-protein interactions. Copyright © 2019 Elsevier Inc. All rights reserved.
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This record was last updated on 08/23/2020 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/31494245