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High throughput approaches to study RNA-protein interactions in vitro.

Authors
  • Ye, Xuan1
  • Jankowsky, Eckhard2
  • 1 Center for RNA Science and Therapeutics, School of Medicine, Case Western Reserve University, Cleveland, OH, United States. , (United States)
  • 2 Center for RNA Science and Therapeutics, School of Medicine, Case Western Reserve University, Cleveland, OH, United States. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Methods
Publisher
Elsevier
Publication Date
Jun 01, 2020
Volume
178
Pages
3–10
Identifiers
DOI: 10.1016/j.ymeth.2019.09.006
PMID: 31494245
Source
Medline
Language
English
License
Unknown

Abstract

To understand the regulation of gene expression it is critical to determine how proteins interact with and discriminate between different RNAs. In this review, we discuss experimental techniques that utilize high throughput approaches to characterize the interactions of proteins with large numbers of RNAs in vitro. We describe the underlying principles for the main methods, briefly discuss their scope and limitations, and outline how insight from the techniques contributes to our understanding of specificity for RNA-protein interactions. Copyright © 2019 Elsevier Inc. All rights reserved.

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