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High-speed tryptic fingerprinting.

Authors
  • Swadesh, J K
Type
Published Article
Journal
BioTechniques
Publication Date
Nov 01, 1990
Volume
9
Issue
5
Identifiers
PMID: 2176508
Source
Medline
License
Unknown

Abstract

Poly(styrene/divinylbenzene) has found use only recently as a high-performance reversed-phase material. There has been relatively little detailed investigation of the properties of poly(styrene/divinylbenzene) in gradient separation of peptides. It was recently found that both the selectivity and peak capacity of poly(styrene/divinylbenzene) are comparable to those of alkyl bonded phases in tryptic mapping. The present work demonstrates that increasing the temperature and flow rate of the mobile phase at constant column inlet pressure increases the efficiency of a poly(styrene/divinylbenzene) column, permitting rapid separations.

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