Affordable Access

High-speed electrophoretic separation of DNA fragments using a short capillary.

Authors
  • Chan, K C
  • Muschik, G M
  • Issaq, H J
Type
Published Article
Journal
Journal of chromatography. B, Biomedical sciences and applications
Publication Date
Jul 18, 1997
Volume
695
Issue
1
Pages
113–115
Identifiers
PMID: 9271135
Source
Medline
License
Unknown

Abstract

Capillary electrophoresis using a replaceable gel buffer was applied to the separation of DNA fragments. A short effective length capillary (1-2 cm) at low electric field allowed the separation of a 20-1000 bp ladder in 1 min. Although similar separation speed was achieved with a longer capillary at high field, the resolution of larger fragments was degraded. The short effective length capillaries were able to separate the wildtype and mutant PCR products of the TGF-beta1 gene in under 45 s.

Report this publication

Statistics

Seen <100 times