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High resolution tumor targeting in living mice by means of multispectral optoacoustic tomography

Authors
  • Buehler, Andreas1
  • Herzog, Eva1
  • Ale, Angelica1
  • Smith, Bradley D2
  • Ntziachristos, Vasilis1
  • Razansky, Daniel1
  • 1 Technische Universität München und Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingoldstädter Landstraße 1, Neuherberg, 85764, Germany , Neuherberg (Germany)
  • 2 University of Notre Dame, Notre Dame Integrated Imaging Facility, Notre Dame, IN, 46556-5670, USA , Notre Dame (United States)
Type
Published Article
Journal
EJNMMI Research
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Apr 01, 2012
Volume
2
Issue
1
Identifiers
DOI: 10.1186/2191-219X-2-14
Source
Springer Nature
Keywords
License
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Abstract

BackgroundTumor targeting is of high clinical and biological relevance, and major efforts have been made to develop molecular imaging technologies for visualization of the disease markers in tissue. Of particular interest is apoptosis which has a profound role within tumor development and has significant effect on cancer malignancy.MethodsHerein, we report on targeting of phosphatidylserine-exposing cells within live tumor allograft models using a synthetic near infrared zinc(II)-dipicolylamine probe. Visualization of the probe biodistribution is performed with whole body multispectral optoacoustic tomography (MSOT) system and subsequently compared to results attained by planar and tomographic fluorescence imaging systems.ResultsCompared to whole body optical visualization methods, MSOT attains remarkably better imaging capacity by delivering high-resolution scans of both disease morphology and molecular function in real time. Enhanced resolution of MSOT clearly showed that the probe mainly localizes in the vessels surrounding the tumor, suggesting that its tumor selectivity is gained by targeting the phosphatidylserine exposed on the surface of tumor vessels.ConclusionsThe current study demonstrates the high potential of MSOT to broadly impact the fields of tumor diagnostics and preclinical drug development.

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