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High-pressure freezing of tissue obtained by fine-needle biopsy.

Authors
  • Hohenberg, H
  • Tobler, M
  • Müller, M
Type
Published Article
Journal
Journal of microscopy
Publication Date
Aug 01, 1996
Volume
183
Issue
Pt 2
Pages
133–139
Identifiers
PMID: 8805825
Source
Medline
License
Unknown

Abstract

High-pressure freezing (HPF) permits adequate cryoimmobilization (without detectable ice crystals after freeze-substitution) of biological tissue up to a thickness of about 200 microns. Until now the preparation of tissue prior to freezing has been unsatisfactory: sizing of the tissue to the required dimensions takes minutes, during which structural alterations must occur. We demonstrate that the use of a fine-needle biopsy technique minimizes tissue damage and guarantees sample dimensions close to the optimal thickness for HPF. The tissue cores can be cryoimmobilized within 40 s of excision.

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