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High performance liquid chromatographic assay for the quantitation of total glutathione in plasma.

Authors
  • Abukhalaf, Imad K
  • Silvestrov, Natalia A
  • Menter, Julian M
  • von Deutsch, Daniel A
  • Bayorh, Mohamed A
  • Socci, Robin R
  • Ganafa, Agaba A
Type
Published Article
Journal
Journal of Pharmaceutical and Biomedical Analysis
Publisher
Elsevier
Publication Date
May 15, 2002
Volume
28
Issue
3-4
Pages
637–643
Identifiers
PMID: 12008143
Source
Medline
Keywords
License
Unknown

Abstract

A simple and widely used homocysteine HPLC procedure was applied for the HPLC identification and quantitation of glutathione in plasma. The method, which utilizes SBDF as a derivatizing agent utilizes only 50 microl of sample volume. Linear quantitative response curve was generated for glutathione over a concentration range of 0.3125-62.50 micromol/l. Linear regression analysis of the standard curve exhibited correlation coefficient of 0.999. Limit of detection (LOD) and limit of quantitation (LOQ) values were 5.0 and 15 pmol, respectively. Glutathione recovery using this method was nearly complete (above 96%). Intra-assay and inter-assay precision studies reflected a high level of reliability and reproducibility of the method. The applicability of the method for the quantitation of glutathione was demonstrated successfully using human and rat plasma samples.

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