High oligomycin concentrations augment 6-keto-PGF1 alpha production in ventricular cardiomyocytes.
- Authors
- Type
- Published Article
- Journal
- Biochimica et Biophysica Acta
- Publisher
- Elsevier
- Publication Date
- Mar 24, 1994
- Volume
- 1211
- Issue
- 3
- Pages
- 283–288
- Identifiers
- PMID: 8130261
- Source
- Medline
- License
- Unknown
Abstract
Incubation of cultured ventricular cardiomyocytes with high oligomycin concentrations (100 micrograms/ml), either alone or combined with 2-deoxyglucose (20 mM), led to the rapid depletion of cellular ATP. Inositol (poly)phosphate production decreased, and 6-keto PGF1 alpha production was increased. In cells depleted of ATP, either by low oligomycin concentrations or by sodium azide, 6-keto PGF1 alpha was not appreciably increased. There was a 25% rise in the release of fatty acids from the sn-2 position in glycerophospholipids. We suggest that oligomycin at high concentrations causes the release of free arachidonic acid from phospholipids either by non-PIP2-specific PLC and DG lipase or by phospholipase D, phosphatidic acid phosphatase and DG lipase. The effect is unrelated to decreased cellular ATP content.