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High oligomycin concentrations augment 6-keto-PGF1 alpha production in ventricular cardiomyocytes.

Authors
  • Pinson, A
  • Zilberman, Y
  • Tirosh, R
  • Trembovler, V
  • Shohami, E
Type
Published Article
Journal
Biochimica et Biophysica Acta
Publisher
Elsevier
Publication Date
Mar 24, 1994
Volume
1211
Issue
3
Pages
283–288
Identifiers
PMID: 8130261
Source
Medline
License
Unknown

Abstract

Incubation of cultured ventricular cardiomyocytes with high oligomycin concentrations (100 micrograms/ml), either alone or combined with 2-deoxyglucose (20 mM), led to the rapid depletion of cellular ATP. Inositol (poly)phosphate production decreased, and 6-keto PGF1 alpha production was increased. In cells depleted of ATP, either by low oligomycin concentrations or by sodium azide, 6-keto PGF1 alpha was not appreciably increased. There was a 25% rise in the release of fatty acids from the sn-2 position in glycerophospholipids. We suggest that oligomycin at high concentrations causes the release of free arachidonic acid from phospholipids either by non-PIP2-specific PLC and DG lipase or by phospholipase D, phosphatidic acid phosphatase and DG lipase. The effect is unrelated to decreased cellular ATP content.

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