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High LET Radiation Overcomes In Vitro Resistance to X-Rays of Chondrosarcoma Cell Lines.

Authors
  • Chevalier, Francois1, 2
  • Hamdi, Dounia Houria1, 2
  • Lepleux, Charlotte1, 2
  • Temelie, Mihaela1, 2, 3
  • Nicol, Anaïs3
  • Austry, Jean Baptiste1
  • Lesueur, Paul4
  • Vares, Guillaume5
  • Savu, Diana2
  • Nakajima, Tetsuo6
  • Saintigny, Yannick1
  • 1 1 CEA GANIL, Caen, France. , (France)
  • 2 2 Horia Hulubei National Institute of Physics and Nuclear Engineering, Bucharest, Romania. , (Oman)
  • 3 3 Centre Paul Strauss, Strasbourg, Alsace, France. , (France)
  • 4 4 Centre Francois Baclesse Centre de Lutte Contre le Cancer, Caen, France. , (France)
  • 5 5 Okinawa Institute of Science and Technology, Kunigami-gun, Okinawa, Japan. , (Japan)
  • 6 6 National Institute of Radiological Sciences, Chiba, Japan. , (Japan)
Type
Published Article
Journal
Technology in Cancer Research & Treatment
Publisher
SAGE Publications
Publication Date
Jan 01, 2019
Volume
18
Identifiers
DOI: 10.1177/1533033819871309
PMID: 31495269
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Chondrosarcomas are malignant tumors of the cartilage that are chemoresistant and radioresistant to X-rays. This restricts the treatment options essential to surgery. In this study, we investigated the sensitivity of chondrosarcoma to X-rays and C-ions in vitro. The sensitivity of 4 chondrosarcoma cell lines (SW1353, CH2879, OUMS27, and L835) was determined by clonogenic survival assays and cell cycle progression. In addition, biomarkers of DNA damage responses were analyzed in the SW1353 cell line. Chondrosarcoma cells showed a heterogeneous sensitivity toward irradiation. Chondrosarcoma cell lines were more sensitive to C-ions exposure compared to X-rays. Using D10 values, the relative biological effectiveness of C-ions was higher (relative biological effectiveness = 5.5) with cells resistant to X-rays (CH2879) and lower (relative biological effectiveness = 3.7) with sensitive cells (L835). C-ions induced more G2 phase blockage and micronuclei in SW1353 cells as compared to X-rays with the same doses. Persistent unrepaired DNA damage was also higher following C-ions irradiation. These results indicate that chondrosarcoma cell lines displayed a heterogeneous response to conventional radiation treatment; however, treatment with C-ions irradiation was more efficient in killing chondrosarcoma cells, compared to X-rays.

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