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High frequency electroporation efficiency is under control of membrane capacitive charging and voltage potential relaxation.

Authors
  • Novickij, Vitalij1
  • Ruzgys, Paulius2
  • Grainys, Audrius3
  • Šatkauskas, Saulius2
  • 1 Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Naugarduko g. 41, 03227 Vilnius, Lithuania. Electronic address: [email protected] , (Lithuania)
  • 2 Biophysical Research Group, Vytautas Magnus University, Vileikos g. 8-212, 44404 Kaunas, Lithuania. , (Lithuania)
  • 3 Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Naugarduko g. 41, 03227 Vilnius, Lithuania. , (Lithuania)
Type
Published Article
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
Publication Date
Feb 01, 2018
Volume
119
Pages
92–97
Identifiers
DOI: 10.1016/j.bioelechem.2017.09.006
PMID: 28922628
Source
Medline
Keywords
License
Unknown

Abstract

The study presents the proof of concept for a possibility to achieve a better electroporation in the MHz pulse repetition frequency (PRF) region compared to the conventional low frequency protocols. The 200ns×10 pulses bursts of 10-14kV/cm have been used to permeabilize Chinese hamster ovary (CHO) cells in a wide range (1Hz-1MHz) of PRF. The permeabilization efficiency was evaluated using fluorescent dye assay (propidium iodide) and flow cytometry. It was determined that a threshold PRF exists when the relaxation of the cell transmembrane potential is longer than the delay between the consequent pulses, which results in accumulation of the charge on the membrane. For the CHO cells and 0.1S/m electroporation medium, this phenomenon is detectable in the 0.5-1MHz range. It was shown that the PRF is an important parameter that could be used for flexible control of electroporation efficiency in the high frequency range.

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