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High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues.

  • You, Shangting
  • Xiang, Yi
  • Hwang, Henry H
  • Berry, David B
  • Kiratitanaporn, Wisarut
  • Guan, Jiaao
  • Yao, Emmie
  • Tang, Min
  • Zhong, Zheng
  • Ma, Xinyue
  • Wangpraseurt, Daniel
  • Sun, Yazhi
  • Lu, Ting-Yu
  • Chen, Shaochen
Publication Date
Feb 01, 2023
eScholarship - University of California
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Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying the requirements of high cell density (HCD), high cell viability, and fine fabrication resolution. In particular, bioprinting resolution of digital light processing-based 3D bioprinting suffers with increasing bioink cell density due to light scattering. We developed a novel approach to mitigate this scattering-induced deterioration of bioprinting resolution. The inclusion of iodixanol in the bioink enables a 10-fold reduction in light scattering and a substantial improvement in fabrication resolution for bioinks with an HCD. Fifty-micrometer fabrication resolution was achieved for a bioink with 0.1 billion per milliliter cell density. To showcase the potential application in tissue/organ 3D bioprinting, HCD thick tissues with fine vascular networks were fabricated. The tissues were viable in a perfusion culture system, with endothelialization and angiogenesis observed after 14 days of culture.

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