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HHV-5 epitope: A potential vaccine candidate with high antigenicity and large coverage.

Authors
  • Kumar, Neeraj1
  • Singh, Aditi2, 3
  • Grover, Sonam4
  • Kumari, Anchala2, 3
  • Kumar Dhar, Pawan3
  • Chandra, Ramesh1
  • Grover, Abhinav3
  • 1 a Drug Discovery and Development Laboratory, Department of Chemistry , University of Delhi , New Delhi , India. , (India)
  • 2 b Department of Biotechnology , TERI School of Advanced Studies , New Delhi , India. , (India)
  • 3 c School of Biotechnology , Jawaharlal Nehru University , New Delhi , India. , (India)
  • 4 d Kusuma School of Biological Sciences , IIT Delhi , New Delhi , India. , (India)
Type
Published Article
Journal
Journal of biomolecular structure & dynamics
Publication Date
May 01, 2019
Volume
37
Issue
8
Pages
2098–2109
Identifiers
DOI: 10.1080/07391102.2018.1477620
PMID: 30044169
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Outbreak of Human Herpes virus-5 (HHV-5) infection in emerging countries has raised worldwide health concern owing to prevalence of congenital impairments and life threatening consequences in immunocompromised individuals. Thus, there lies an impending need to develop vaccine against HHV-5. HHV-5 enters into host cells with the help of necessary components glycoprotein B (gB) and H/L. In this study, the conformational linear B-cell and T-cell epitopes for gB of HHV-5 have been predicted using conformational approaches, for their possible collective use as vaccine candidates. We examined epitope's interactions with major histocompatibility complexes using molecular docking and also investigated their stable binding with specific toll like receptor-2 (TLR2), present on host cells during HHV-5 infection. Predicted MHC-I epitope 'LVAIAVVII' with high antigenicity and large coverage of HLA alleles was found to superimpose on MHC-II epitope (Rank 1) and was also identified to be the core sequence of putative B cell epitope 'ILVAIAVVIITYLI'. Resulting epitope was found to have consistent interaction with TLR2 during long term (100 ns) MD run. We also validated this nonamer epitope for its dissimilarity with human genome and high population coverage, suggesting it to be a potential vaccine candidate with higher coverage for both the MHC alleles of Indian population. Communicated by Ramaswamy H. Sarma.

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