Affordable Access

Heterologous activation of the actinorhodin biosynthetic pathway in Streptomyces lividans.

Authors
  • N M Romero
  • V Parro
  • F Malpartida
  • R P Mellado
Publication Date
Jun 11, 1992
Source
PMC
License
Unknown

Abstract

A DNA fragment of Streptomyces fradiae is able to activate the antibiotic actinorhodin biosynthetic pathway when cloned in Streptomyces lividans. The activator DNA region has been sequenced and its transcription initiation and termination sites accurately mapped in vivo. This DNA encodes a 132 nucleotides long transcript which is apparently responsible for the actinorhodin production phenotype, possibly acting as an antisense RNA. The sequence of the activator gene revealed no homology with any other known Streptomyces coelicolor genes concerned with actinorhodin biosynthesis or its pleiotropic regulation.

Report this publication

Statistics

Seen <100 times