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Heterogeneity of the Native Ribosomal Subunit Fraction in HeLa Cells

Authors
  • Lecou, Christian
  • Arends, Sigrid
  • Liautard, Jean-Pierre
  • Köhler, Kurt
Type
Published Article
Journal
Zeitschrift für Naturforschung C
Publisher
Walter de Gruyter GmbH
Publication Date
Apr 01, 1973
Volume
28
Issue
3-4
Pages
183–187
Identifiers
DOI: 10.1515/znc-1973-3-415
Source
De Gruyter
Keywords
License
Green

Abstract

The cytoplasm of growing HeLa cells contains a certain quantity of native ribosomal subunits. They constitute a heterogeneous fraction consisting of at least two categories of particles. The first category comprises the newly emerging native 40S subunits which do not form 80S couples under any in vitro condition tried. It is postulated that these native subunits carry an anti-association factor which cannot be stripped off by 0.5 м KCl treatment. Transiently native subunits of the second category reassociate to 80S ribsomes only in the presence of an association factor.

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