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Herbaspirillum rubrisubalbicans as a phytopathogenic model to study the immune system of Sorghum bicolor.

Authors
  • Tuleski, Thalita Regina1
  • Kimball, Jennifer2
  • Amaral, Fernanda Plucani do3
  • Pereira, Tomas4
  • Tadra-Sfeir, Michelle5
  • Pedrosa, Fabio de Oliveira6
  • Souza, Emanuel Maltempi7
  • Balint-Kurti, Peter8, 9
  • Monteiro, Rose Adele10
  • Stacey, Gary11
  • 1 Universidade Federal do Parana, 28122, Biochemistry and Molecular Biology, Francisco H. dos Santos, Centro Politécnico - Jardim das Américas, CEP: 81531-980, Curitiba, Parana, Brazil, 81531-980; [email protected] , (Brazil)
  • 2 North Carolina State University, Department of Entomology and Plant Pathology, Raleigh, North Carolina, United States; [email protected] , (United States)
  • 3 University of Missouri, Divisions of Plant Science and Biochemistry, Columbia, Missouri, United States; [email protected] , (United States)
  • 4 Federal University of Santa Catarina, Department of Department of Science and Food Technology, Florianopolis, Santa Catarina, Brazil; [email protected] , (Brazil)
  • 5 Universidade Federal do Parana, 28122, Biochemistry and Molecular Biology, Curitiba, Parana, Brazil; [email protected] , (Brazil)
  • 6 Rua Francisco H. dos Santos s/nCuritiba, Paraná, Brazil, 81531 980; [email protected] , (Brazil)
  • 7 UFPR, Biochemistry and Molecular Biology, Curitiba, Parana, Brazil; [email protected] , (Brazil)
  • 8 North Carolina State University, Entomology and Plant Pathology, Raleigh, North Carolina, United States. , (United States)
  • 9 USDA-ARS, Plant Science Research Unit, Raleigh, North Carolina, United States; [email protected] , (United States)
  • 10 UFPR, Biochemistry and Molecular Biology, Rua Francisco H dos Santos S/N, Jardim das Américas Curitiba - Paraná - Brasil, CEP 81531-990 CxPostal 19046, Curitiba, Parana, Brazil, 19046; [email protected] , (Brazil)
  • 11 University of Missouri, Department of Plant Microbiology and Pathology, 271E Life Science Center, Columbia, Missouri, United States, 65211; [email protected] , (United States)
Type
Published Article
Journal
Molecular Plant-Microbe Interactions
Publisher
Scientific Societies
Publication Date
Nov 13, 2019
Identifiers
DOI: 10.1094/MPMI-06-19-0154-R
PMID: 31721651
Source
Medline
Language
English
License
Unknown

Abstract

Herbaspirillum rubrisubalbicans is the causal agent of red stripe disease (RSD) and mottle stripe disease of sorghum and sugar cane, respectively.Sixty-three genotypes of Sorghum bicolor were inoculated with H. rubrisubalbicans with 59 showing RSD symptoms. QTL analysis in a recombinant inbred Line (RIL) population identified several quantitative trait loci (QTL) associated with variation in resistance to RSD. RNA sequencing analysis identified a number of genes whose transcript levels were differentially regulated during H. rubrisubalbicans infection. Among those genes that responded to H. rubrisubalbicans inoculation were many known to be involved in plant-pathogen interactions, such as leucine-rich (LRR) receptors, calcium binding proteins, transcriptional factors (ERF) and, callose synthase. Pretreatment of sorghum leaves with the pathogen-associated molecular pattern (PAMP) molecules flg22 and chitooctaose provided protection against subsequent challenge with the pathogen, suggesting that PAMP-triggered immunity (PTI) plays an important role in the sorghum immunity response. These data present baseline information for the use of the genetically tractable H. rubrisubalbicans-sorghum pathosystem for the study of innate immunity and disease resistance in this important grain and bioenergy crop. Information gained from the use of this system is likely to be informative for other monocots, including those more intractable for experimental study (e.g., sugarcane).

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