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Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles

  • Hwang, Yong Hwa1
  • Kim, Youn-Jung2, 3
  • Lee, Dong Yun1, 4
  • 1 College of Engineering, and BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, Institute of Nano Science & Technology (INST), Hanyang University, Seoul, 04763, Republic of Korea , Seoul (South Korea)
  • 2 College of Natural Sciences, Incheon National University, Incheon, 22012, Republic of Korea , Incheon (South Korea)
  • 3 Incheon National University, Incheon, 22012, Republic of Korea , Incheon (South Korea)
  • 4 Elixir Pharmatech Inc., Seoul, 04763, Republic of Korea , Seoul (South Korea)
Published Article
Biomaterials Research
Springer (Biomed Central Ltd.)
Publication Date
Nov 04, 2021
DOI: 10.1186/s40824-021-00241-7
Springer Nature
  • Research Article


BackgroundSuperparamagnetic iron oxide (SPIO) nanoparticles have been widely used in several biomedical engineering in vivo. Although various surface modifications have been made to these non-biodegradable nanoparticles to make them more biocompatible, their toxic potential still remains a major concern.MethodIn this study, we newly developed unfractionated heparin (UFH)-coated and low molecular weight heparin (LMWH)-coated SPIO nanoparticles through surface modification engineering, which was compared with commercially available dextran-coated SPIO nanoparticles. Their toxicity such as cytotoxicity, single cell gel electrophoresis (SCGE) comet assay, intracellular reactive oxygen species (ROS) content and cellular apoptosis was evaluated to hepatic HepG2 and renal HK-2 cells.ResultsWhen UFH-, LMWH- or dextran-coated SPIO nanoparticles were applied, they did not affect the viability of HepG2 cell. However, HK-2 cells were more sensitive to dextran-coated SPIO nanoparticles than others. In genotoxicity assay using SCGE comet, DNA tail moment values in the groups treated with dextran- and LMWH-coated SPIO nanoparticles significantly increased. However, UFH-coated SPIO nanoparticles was only significantly lowing DNA tail moment value. In addition, UFH-coated SPIO nanoparticles had lower cytotoxicity in HepG2 and HK-2 cells compared to dextran-coated SPIO nanoparticles, especially in terms of apoptosis and intracellular ROS production.ConclusionsCollectively, it is possible that UFH- coated SPIO nanoparticles can be used as alternative negative contrast agents.

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