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Hepatic gene expression changes in an experimental model of accelerated senescence: the SAM-P8 mouse.

Authors
  • Vilà, Laia
  • Roglans, Núria
  • Alegret, Marta
  • Camins, Antoni
  • Pallàs, Mercè
  • Sánchez, Rosa María
  • Vázquez-Carrera, Manuel
  • Laguna, Juan Carlos
Type
Published Article
Journal
The journals of gerontology. Series A, Biological sciences and medical sciences
Publication Date
Oct 01, 2008
Volume
63
Issue
10
Pages
1043–1052
Identifiers
PMID: 18948554
Source
Medline
License
Unknown

Abstract

The senescence-accelerated mouse (SAM) is an experimental model of aging, established through phenotypic selection from a common genetic pool of AKR/J mice. Here we use complementary DNA microarray, Western blot, and electrophoretic mobility shift assay to consider whether changes in liver gene expression observed in 5-month-old SAM-prone 8 (P8) mice, compared to SAM-R1 controls, are similar to those reported in aged rodents. Livers from SAM-P8 mice presented 88 differentially expressed transcripts, 59% of which were upregulated and 41% were downregulated. Of these, 14% were related to inflammatory/immunity processes, 10% were related to the xenobiotic metabolism (XM) and 3% to nervous system pathophysiology (NSP). Depressed expression and activity of genes related to XM, and altered expression of genes related to NSP, are similar to changes observed in aged rodents. Increased expression of heat shock protein 1 and Jun-B, reduced activity of activator protein 1 and absence of nuclear factor-kappaB activation indicate the lack of a strong liver inflammatory response in 5-month-old SAM-P8 mice.

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