Affordable Access

Hemoglobin switching in sheep and goats: change in functional globin messenger RNA in reticulocytes and bone marrow cells.

Authors
  • Nienhuis, A W
  • Anderson, W F
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Aug 01, 1972
Volume
69
Issue
8
Pages
2184–2188
Identifiers
PMID: 4506088
Source
Medline
License
Unknown

Abstract

ANEMIA CAUSES A CHANGE IN THE TYPE OF CIRCULATING HEMOGLOBIN IN GOATS AND CERTAIN SHEEP: HbA (alpha(2)beta(2) (A)) is replaced by HbC (alpha(2)beta(2) (C)). We have isolated globin mRNA from erythroid cells of anemic and nonanemic animals to investigate the mechanism whereby anemia causes this switch. To study several stages in transition from beta(A) to beta(C) synthesis, active globin mRNA was isolated from bone marrow cells, as well as from reticulocytes. By assaying these globin mRNAs in a rabbit reticulocyte cell-free system, we have demonstrated that the switch from beta(A) to beta(C) globin synthesis is mediated via a change in functional globin mRNA.

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