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Heat shock protein HSP90 immunoexpression in equine endometrium during oestrus, dioestrus and anoestrus.

Authors
  • Camacho Benítez, Ana1
  • Vasconcellos, Rossana1
  • Lombide, Paula1
  • Viotti, Helen1
  • Pérez, William2
  • Cazales, Nicolás3
  • Cavestany, Daniel3
  • Martin, Graeme B4, 5
  • Pedrana, Graciela1
  • 1 Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. , (Uruguay)
  • 2 Anatomía, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. , (Uruguay)
  • 3 Centro de Posgrados, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. , (Uruguay)
  • 4 School of Agriculture and Environment, University of Western Australia, Crawley, WA, Australia. , (Australia)
  • 5 Faculty of Science, UWA School of Agriculture and Environment, University of Western Australia, Perth, Australia. , (Australia)
Type
Published Article
Journal
Anatomia Histologia Embryologia
Publisher
Wiley (Blackwell Publishing)
Publication Date
Jan 01, 2021
Volume
50
Issue
1
Pages
50–57
Identifiers
DOI: 10.1111/ahe.12598
PMID: 32776605
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Heat shock proteins play a crucial role in cellular development, proliferation, differentiation and apoptosis. Heat shock protein 90 (HSP90) has been localised in the human endometrium, where its immunoexpression changes during the menstrual cycle. Similar studies have not been done for the equid species, so the present study aimed to describe endometrial HSP90 immunoexpression in mare endometrium. Endometrial biopsies were formalin-fixed and paraffin-embedded, and sections were stained with haematoxylin-eosin in preparation for HSP90 immunohistochemistry. Immunostaining and morphometric analyses were performed on the epithelial lining, endometrial glands and connective stroma during oestrus, dioestrus phase and anoestrus period (n = 7 per phase or period). Immunoexpression was localised in the basal region of the epithelial cells lining the lumen. Immunoexpression was greater during oestrus than during either dioestrus or anoestrus. During anoestrus, there was little immunostaining in the endometrium, suggesting that HSP90 is involved in the functional modulation of sex steroid receptors in cyclic mares. Indeed, the function of HSP90 as a chaperone in the folding of proteins, such as steroid receptors, might explain the greater intensity of immunostaining during the oestrus and dioestrus phases, compared the anoestrus period. We conclude that, in the mare, HSP90 plays a role in endometrial function and that further studies are needed to test whether it is important in pathological conditions as endometritis. © 2020 Wiley-VCH GmbH.

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