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Heat-shock protein 70 PCR-RFLP: a universal simple tool for Leishmania species discrimination in the New and Old World.

Authors
  • Montalvo, A M
  • Fraga, J
  • Monzote, L
  • Montano, I
  • De Doncker, S
  • Dujardin, J C
  • Van der Auwera, G
Type
Published Article
Journal
Parasitology
Publication Date
Jul 01, 2010
Volume
137
Issue
8
Pages
1159–1168
Identifiers
DOI: 10.1017/S0031182010000089
PMID: 20441679
Source
Medline
License
Unknown

Abstract

Currently no assay is available for global Leishmania species discrimination. We present a universal PCR-RFLP method allowing identification of most medically relevant Old and New World Leishmania species on the basis of a single PCR, obviating the need to perform separate PCRs. The technique is simple to perform and can be implemented in all settings where PCR is available.

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