The aim of this study was to analyze the health and wellness status perception in amateur half-marathon runners according to sex, age, being injured or not during the two months prior to the race, and having the support or not of qualified staff for race preparation. Six hundred and twenty-four amateur level half-marathon athletes (515 men and 107 women; 41.5 ± 10.1 years) participated in the study. One week before competing in a half-marathon, participants answered the Hooper Index and the SF-36 questionnaire. Women stated higher stress before competing in the race ( p < 0.01) compared to men and the group of runners of <40 years stated greater fatigue ( p < 0.05) compared to the group of >40 years. Women showed a better quality of life in physical and emotional role dimensions ( p < 0.05), and the group of >40 years showed a better quality of life in the emotional role dimension ( p < 0.05). The group that had suffered an injury (InjuryYes) declared greater muscle soreness (MusclSore; p < 0.01), and the group that had qualified staff (QualifStaffYes) declared a higher level of stress ( p < 0.05) and fatigue ( p < 0.01). The Injury No (InjuryNo) group showed a better quality of life in the physical function dimension ( p < 0.01). The group that did not have qualified staff (QualifStaffNo) showed a better quality of life in the dimensions of body pain, general health, vitality, social function ( p < 0.05), and mental health ( p < 0.01), while the QualifStaffYes group showed better results in the dimensions of physical function and emotional role ( p < 0.05). Sex, age, being injured or not during the two months prior to the race, and having the support or not of qualified staff for the race preparation can influence the health and wellness status perception.