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HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.

Authors
  • Gemoll, Timo1
  • Roblick, Uwe J
  • Szymczak, Silke
  • Braunschweig, Till
  • Becker, Susanne
  • Igl, Bernd-Wolfgang
  • Bruch, Hans-Peter
  • Ziegler, Andreas
  • Hellman, Ulf
  • Difilippantonio, Michael J
  • Ried, Thomas
  • Jörnvall, Hans
  • Auer, Gert
  • Habermann, Jens K
  • 1 Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden. , (Sweden)
Type
Published Article
Journal
Cellular and Molecular Life Sciences
Publisher
Springer-Verlag
Publication Date
Oct 01, 2011
Volume
68
Issue
19
Pages
3261–3274
Identifiers
DOI: 10.1007/s00018-011-0628-3
PMID: 21290163
Source
Medline
License
Unknown

Abstract

DNA aneuploidy has been identified as a prognostic factor for epithelial malignancies. Further understanding of the translation of DNA aneuploidy into protein expression will help to define novel biomarkers to improve therapies and prognosis. DNA ploidy was assessed by image cytometry. Comparison of gel-electrophoresis-based protein expression patterns of three diploid and four aneuploid colorectal cancer cell lines detected 64 ploidy-associated proteins. Proteins were identified by mass spectrometry and subjected to Ingenuity Pathway Analysis resulting in two overlapping high-ranked networks maintaining Cellular Assembly and Organization, Cell Cycle, and Cellular Growth and Proliferation. CAPZA1, TXNL1, and HDAC2 were significantly validated by Western blotting in cell lines and the latter two showed expression differences also in clinical samples using a tissue microarray of normal mucosa (n=19), diploid (n=31), and aneuploid (n=47) carcinomas. The results suggest that distinct protein expression patterns, affecting TXNL1 and HDAC2, distinguish aneuploid with poor prognosis from diploid colorectal cancers.

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