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Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora.

Authors
  • Wei, Z M
  • Laby, R J
  • Zumoff, C H
  • Bauer, D W
  • He, S Y
  • Collmer, A
  • Beer, S V
Type
Published Article
Journal
Science (New York, N.Y.)
Publication Date
Jul 03, 1992
Volume
257
Issue
5066
Pages
85–88
Identifiers
PMID: 1621099
Source
Medline
License
Unknown

Abstract

A proteinaceous elicitor of the plant defense reaction known as the hypersensitive response was isolated from Erwinia amylovora, the bacterium that causes fire blight of pear, apple, and other rosaceous plants. The elicitor, named harpin, is an acidic, heat-stable, cell-envelope-associated protein with an apparent molecular weight of 44 kilodaltons. Harpin caused tobacco leaf lamina to collapse and caused an increase in the pH of bathing solutions of suspension-cultured tobacco cells. The gene encoding harpin (hrpN) was located in the 40-kilobase hrp gene cluster of E. amylovora, sequenced, and mutated with Tn5tac1. The hrpN mutants were not pathogenic to pear, did not elicit the hypersensitive response, and did not produce harpin.

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