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Harnessing glycomics technologies: integrating structure with function for glycan characterization.

Authors
  • Robinson, Luke N
  • Artpradit, Charlermchai
  • Raman, Rahul
  • Shriver, Zachary H
  • Ruchirawat, Mathuros
  • Sasisekharan, Ram
Type
Published Article
Journal
Electrophoresis
Publisher
Wiley (John Wiley & Sons)
Publication Date
Mar 01, 2012
Volume
33
Issue
5
Pages
797–814
Identifiers
DOI: 10.1002/elps.201100231
PMID: 22522536
Source
Medline
License
Unknown

Abstract

Glycans, or complex carbohydrates, are a ubiquitous class of biological molecule which impinge on a variety of physiological processes ranging from signal transduction to tissue development and microbial pathogenesis. In comparison to DNA and proteins, glycans present unique challenges to the study of their structure and function owing to their complex and heterogeneous structures and the dominant role played by multivalency in their sequence-specific biological interactions. Arising from these challenges, there is a need to integrate information from multiple complementary methods to decode structure-function relationships. Focusing on acidic glycans, we describe here key glycomics technologies for characterizing their structural attributes, including linkage, modifications, and topology, as well as for elucidating their role in biological processes. Two cases studies, one involving sialylated branched glycans and the other sulfated glycosaminoglycans, are used to highlight how integration of orthogonal information from diverse datasets enables rapid convergence of glycan characterization for development of robust structure-function relationships.

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