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gyrA mutations in quinolone-resistant isolates of the fish pathogen Aeromonas salmonicida.

Authors
  • H Oppegaard
  • H Sørum
Publication Date
Oct 01, 1994
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

gyrA mutations in quinolone-resistant isolates of Aeromonas salmonicida have been detected by using PCR to amplify the quinolone resistance-determining region of gyrA and subsequent cloning and sequencing of PCR products. Comparison of nucleotide and derived amino acid sequences of PCR products from quinolone-susceptible and -resistant bacteria revealed a serine 83-to-isoleucine substitution in the gyrase A protein of resistant isolates. One of the resistant isolates differed from the other by a two- to fourfold-higher MIC of the fluoroquinolone enrofloxacin and carried an additional alanine 67-to-glycine substitution, which may contribute to the higher level of resistance.

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