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Gut microbiota and metabonomics used to explore the mechanism of Qing'e Pills in alleviating osteoporosis.

Authors
  • Xie, Hui1, 2
  • Hua, Zhengying1, 2
  • Guo, Mengyu1, 2
  • Lin, Shangyang1, 2
  • Zhou, Yaqian1, 2
  • Weng, Zebin3
  • Wu, Li1
  • Chen, Zhipeng1, 2
  • Xu, Zisheng4
  • Li, Weidong1, 2
  • 1 School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. , (China)
  • 2 Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China. , (China)
  • 3 School of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, China. , (China)
  • 4 Wuhu Pure Sunshine Natural Medicine Company Limited, Wuhu, China. , (China)
Type
Published Article
Journal
Pharmaceutical Biology
Publisher
Informa UK (Taylor & Francis)
Publication Date
Dec 01, 2022
Volume
60
Issue
1
Pages
785–800
Identifiers
DOI: 10.1080/13880209.2022.2056208
PMID: 35387559
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The traditional Chinese medicine Qing'e Pills (QEP) has been used to treat postmenopausal osteoporosis (PMO). We evaluated the regulatory effects of QEP on gut microbiota in osteoporosis. Eighteen female SD rats were divided into three groups: sham surgery (SHAM), ovariectomized (OVX) and ovariectomized treated with QEP (OVX + QEP). Six weeks after ovariectomy, QEP was administered to OVX + QEP rats for eight weeks (4.5 g/kg/day, i.g.). After 14 weeks, the bone microstructure was evaluated. Differences in gut microbiota were analysed via 16S rRNA gene sequencing. Changes in endogenous metabolites were studied using UHPLC-Q-TOF/MS technology. GC-MS was used to detect short-chain fatty acids. Furthermore, we measured serum inflammatory factors, such as IL-6, TNF-α and IFN-γ, which may be related to gut microbiota. OVX + QEP exhibited increased bone mineral density (0.11 ± 0.03 vs. 0.21 ± 0.02, p< 0.001) compared to that of OVX. QEP altered the composition of gut microbiota. We identified 19 potential biomarkers related to osteoporosis. QEP inhibited the elevation of TNF-α (38.86 ± 3.19 vs. 29.43 ± 3.65, p< 0.05) and IL-6 (83.38 ± 16.92 vs. 45.26 ± 3.94, p< 0.05) levels, while it increased the concentrations of acetic acid (271.95 ± 52.41 vs. 447.73 ± 46.54, p< 0.001), propionic acid (28.96 ± 5.73 vs. 53.41 ± 14.26, p< 0.01) and butyric acid (24.92 ± 18.97 vs. 67.78 ± 35.68, p< 0.05). These results indicate that QEP has potential of regulating intestinal flora and improving osteoporosis. The combination of anti-osteoporosis drugs and intestinal flora could become a new treatment for osteoporosis.

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