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Gustatory receptor 28b is necessary for avoiding saponin in Drosophila melanogaster.

Authors
  • Sang, Jiun1
  • Rimal, Suman1
  • Lee, Youngseok2
  • 1 Department of Bio and Fermentation Convergence Technology, Kookmin University BK21 PLUS project, Seoul, Korea. , (North Korea)
  • 2 Department of Bio and Fermentation Convergence Technology, Kookmin University BK21 PLUS project, Seoul, Korea [email protected] , (North Korea)
Type
Published Article
Journal
EMBO Reports
Publisher
EMBO
Publication Date
Feb 01, 2019
Volume
20
Issue
2
Identifiers
DOI: 10.15252/embr.201847328
PMID: 30622216
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Saponins function as a natural self-defense mechanism for plants to deter various insects due to their unpleasant taste and their toxicity. Here, we provide evidence that saponin from Quillaja saponaria functions as an antifeedant as well as an insecticide to ward off insects in both the larval and the adult stages. Using a behavioral screen of 26 mutant fly lines, we show that the Gr28b gene cluster plays a role in saponin avoidance in the labellum. The Gr28b mutant does not avoid saponin and exhibits increased lethality when fed saponin-mixed food. Tissue-specific rescue experiments with five different Gr28b isoforms revealed that only the Gr28b.c isoform is required for saponin sensation. We propose that in contrast to sensing many other bitter compounds, saponin sensing does not require the function of core taste receptors, such as GR32a, GR33a, and GR66a. Our results reveal a novel role for GR28b in taste. In addition, the ability of saponin to act as insecticides as well as antifeedants suggests its potential application in controlling insect pests. © 2019 The Authors.

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